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+//Harriot P.,2003,Chemical Reactor Design (I-Edition) Marcel Dekker,Inc.,USA,pp 436.
+//Chapter-1 Ex1.5 Pg No. 29
+//Title: Methods to determine km and vm
+//========================================================================================
+clear
+clc
+clf
+//INPUT
+S=[2;5;10;15]*10^(-3);//Concentration of substrate [HCO3]
+r_reciprocal=[95;45;29;25]*10^(3);//Reciprocal rates (L-sec/mol)
+
+//CALCULATION
+//Plot 1 refer equation 1.24 Pg No.29
+x1=(S).^(-1);
+y1=r_reciprocal;
+scf(0)
+plot(x1,y1*10^(-3),'RED');
+xlabel("1/[S]");
+ylabel("(1/r)*10^-3");
+xtitle("1/r versus 1/S");
+p=length(x1);
+X_1=[x1 ones(p,1)];
+R1=X_1\y1;
+slope(1)=R1(1,1);
+intercept(1)=R1(2,1);
+v_m(1)=(1/(intercept(1)));//Maximum Reaction Rate(mol/L-sec)
+k_m(1)=slope(1)*v_m(1);//Michaelis-Menton constant
+
+//Plot 2 refer equation 1.25 Pg No.29
+x2=S;
+y2=S.*r_reciprocal;
+scf(1)
+plot(x2*10^(3),y2);
+xlabel("(S)*10^3");
+ylabel("(S)/r");
+xtitle("(S)/r versus (S)");
+q=length(x2);
+X_2=[x2 ones(q,1)];
+R2=X_2\y2;
+slope(2)=R2(1,1);
+intercept(2)=R2(2,1);
+v_m(2)=1/(slope(2));//Maximum Reaction Rate (mol/L-sec)
+k_m(2)=intercept(2)/(slope(2));//Michaelis-Menton constant
+
+
+//OUTPUT
+mprintf('\n======================================================================================');
+mprintf('\n \t\tMethod_1\tMethod_2');
+mprintf('\n======================================================================================');
+i=1
+ mprintf('\n Slope \t%f\t%f',slope(i),slope(i+1));
+ mprintf('\n Intercept \t%f\t%f',intercept(i),intercept(i+1));
+ mprintf('\n Km (M) \t%f\t%f',k_m(i),k_m(i+1));
+ mprintf('\n Vm(mol/L-sec) %f\t%f',v_m(i),v_m(i+1));
+
+//FILE OUTPUT
+fid= mopen('.\Chapter1-Ex5-Output.txt','w');
+mfprintf(fid,'\n======================================================================================');
+mfprintf(fid,'\n \t\tMethod_1\tMethod_2');
+mfprintf(fid,'\n======================================================================================');
+i=1
+ mfprintf(fid,'\n Slope \t%f\t%f',slope(i),slope(i+1));
+ mfprintf(fid,'\n Intercept \t%f\t%f',intercept(i),intercept(i+1));
+ mfprintf(fid,'\n Km (M) \t%f\t%f',k_m(i),k_m(i+1));
+ mfprintf(fid,'\n Vm(mol/L-sec) %f\t%f',v_m(i),v_m(i+1));
+mclose(fid);
+
+//========================================================================END OF PROGRAM=================================
+//Disclaimer: Least Square method is used to find the slope and intercept in this example.
+// Hence the values differ from the graphically obtained values of slope and intercept in the textbook.
+
+
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